动物行为与生理核心|概述
*动物行为和生理核心现已开放。请查看我们的新新冠肺炎standard operating procedures在预订或到达核心之前。
Our mission
The Animal Behavior and Physiology (AB&P) Core is designed to provide a comprehensive state-of-the-art in vivo preclinical, translational platform for in-depth phenotyping of models of neurodevelopmental disorders, development of reliable and quantitative biomarkers, and to measure the effects of therapeutic interventions. AB&P leadership and expanded research team includes expertise in translational neuroscience, rodent behavioral and physiological metrics, drug testing, biomedical engineering, and in core management to facilitate efficient planning, execution and analyses of experimental data.
We recognize the need to enhance and integrate multilevel phenotyping capabilities used for characterizing our animal models both at the behavioral and physiological level at Boston Children’s Hospital within one rodent phenotyping core. The AB&P has been serving investigators since 2011 and has contributed to the publication of over 50 manuscripts in peer-reviewed journals and provided facilities for scientists to obtain data to support numerous successful grant applications. In 2019, the AB&P was recognized for excellence and broad appeal across multiple domains by being made an Institutional Core Facility at Boston Children’s.
AB＆P高级技术
The AB&P facilities include a wide range of advanced technologies for acquiring data at single cell, network and the behavioral level. Such technologies can be combined in the majority of cases in conscious freely behaving rodents, allowing top-down and bottom-up analysis. By design, each available technology is modular with capacity for upgrade, thus minimizing risk of obsolescence in coming years. The behavioral and physiological assays of the AB&P are designed to enable deep model phenotyping, and early phase CNS drug discovery and development by collection and analysis of in vivo behavioral and physiological signals. Services that provide a high-throughput platform for preclinical neurophysiological monitoring to support translational research are absent within the greater Boston area (inclusive of industry facilities). The AB&P thus addresses a conspicuous, unmet need and fosters Boston Children's collaboration with industry and academia both in the Boston area and nationally.
正如设备页面上逐项列出的那样，设备包括独特的模块化平台，可实现多层，全面的神经发育模型模型表型。同样，这些测定法还提供了药理学剂的目标参与度量，并在任何可用模型中都部署了治疗性后，可以实现必要的临床前PK/PD研究。
Our offerings
具体而言，AB＆P核心的独特功能包括：
- ability to house mice in customized modified environments
- 24箱无线视频遥测设置，用于连续记录脑电图，EMG，EKG，Actigraphy，体温和其他指标
- a setup for rodent transcranial magnetic stimulation (TMS)
- 通过多纤维光度法以及行为分析（例如操作行为，社交或焦虑测试）的体内钙成像，
- 在有意识的，自由移动的啮齿动物中，用于对周围和中枢神经系统的神经化学物质和其他成分进行采样的体内微透析。
Notably, AB&P measures are by design translational, and available in human studies at Boston Children's for immediate extension of hypotheses formulated in the preclinical AB&P studies to clinical trials.